Preliminary Note on the Structure of Ascorbic Acid (vitamin C).

in each case varied during the succeeding days and the general attitude varied from extreme lassitude and drowsiness on the one hand to hyperesthesia and clonic spasms on the other. The height of the clinical reaction occurred on the 7th to 9th days, retrogressing to normalcy on the 14th day with the animals remaining normal on the 20th day. Using a very active sample of the same virus, White Leghorn cockerels (3) about 4 months of age and mature common pigeons (4) were inoculated intracerebrally while under light chloroform anesthesia. A 0.2 cc dose of approximately a 1/50 dilution of virulent guinea pig brain was used. The cockerels have remained normal for 20 days. The four pigeons so inoculated developed general weakness, ataxia and marked tremors on the third day; dying on the third to fourth day after inoculation. The brains of these pigeons produced the typical disease in guinea pigs. Pigeons receiving normal guinea pig brain were in no way affected by the inoculation. These inoculations, though too limited for final conclusions, indicate that calves are not entirely refractory to encephalomyelitis. A 100 per cent. "take" in the ease of pigeons indicates the possible use of these birds in routine examination of tissues from field cases. The possible relationship of the calf and the pigeon to the epizootiology of the natural disease is worthy of consideration. L. T. GILTNER M. S. SHAHAN BUREAU OF ANIMAL INDUSTRY U. S. DEPARTMENT OF AGRICULTURE